A novel carrier-free nanoparticle with stable distinctive three-dimensional structure for tumor-targeted precision chemoimmunotherapy.

Publication date: Jul 01, 2025

Despite significant advancements in oncology, cancer remains a leading global health burden, necessitating innovative therapeutic strategies. Here, we present a novel carrier-free tumor-targeted nanomedicine system (DPA NPs) for tumor-targeted chemoimmunotherapy, formed by self-assembly of a conjugate synthesized with doxorubicin (DOX), tumor-homing peptide iRGD, matrix metalloproteinase 2 enzyme responsive peptide (MMP2), and adjuvant monophosphoryl lipid A (MPLA). The results demonstrated that DPA NPs exhibited a stable unique 3D nanostructure with tumor microenvironment (TME)-responsive properties. DPA NPs could efficiently deliver DOX to tumor cells, inducing immunogenic cell death (ICD) and simultaneously triggering tumor specific immune response. Meanwhile, MPLA amplified the anti-tumor immunity, significantly inhibiting tumor growth and metastasis. When combined with immune checkpoint inhibitors (ICIs), DPA NPs further enhanced the therapeutic outcomes in a B16 melanoma model, demonstrating remarkable suppression of tumor growth, metastasis inhibition and recurrence prevention. Mechanistic investigations across multiple biological hierarchies conclusively further confirmed the synergistic therapeutic effect. This study demonstrated that DPA NPs provide a precise, multifunctional nanoplatform for tumor-targeted combination therapy, highlighting their potential for clinical translation in cancer treatment.

Concepts Keywords
Death Animals
Enhanced Antineoplastic Agents
Metalloproteinase Antineoplastic Agents
Nanobiotechnology Cell Line, Tumor
Oncology Combination therapy
Doxorubicin
Doxorubicin
Drug Carriers
Drug Carriers
Female
Humans
Immunogenic cell death
Immunotherapy
Immunotherapy
Lipid A
Lipid A
Matrix Metalloproteinase 2
Matrix Metalloproteinase 2
Melanoma, Experimental
Mice
monophosphoryl lipid A
Nanoparticles
Nanovaccine
Oligopeptides
Oligopeptides
Tumor Microenvironment

Semantics

Type Source Name
disease MESH tumor
drug DRUGBANK Valproic Acid
drug DRUGBANK Doxorubicin
disease MESH metastasis
disease MESH B16 melanoma
disease MESH recurrence
pathway REACTOME Translation

Original Article

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