Publication date: Jul 03, 2025
Mayaro virus (MAYV) is a mosquito-borne alphavirus that is widespread in the Amazon basin, where it co-circulates with the closely related chikungunya virus (CHIKV). Due to the limited surveillance and technical limitations of diagnostic assays (scarcity of commercial assays, serology cross-reactivity), the true burden of MAYV is uncertain. We designed a new RT-qPCR assay targeting the nsp1 gene for MAYV detection, which can be used in monoplex or duplex format. In the duplex format, the new MAYV assay is combined with a CHIKV assay and a second MAYV assay, both previously published. The lower limit of detection with a 95% positivity rate was determined to be
| Concepts | Keywords |
|---|---|
| Alphavirus | Alphavirus |
| Amazon | arbovirus |
| Duplex | chikungunya virus |
| Microbiol | diagnostic |
| Mosquito | Mayaro virus |
| RT-qPCR | |
| Togaviridae |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | assay |
Original Article
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