Non-canonical PRC1.1 licenses transcriptional response to enable Treg plasticity in immune adaptation.

Publication date: Jul 03, 2025

Polycomb repressive complexes (PRCs) sustain regulatory T (Treg) cell identity through transcriptional silencing, yet their role in modulating Treg functional plasticity during immune adaptation remains unclear. Here, we identify KDM2B, a defining component of non-canonical PRC1. 1, as a critical regulator for sustaining the proportion and immunosuppressive functions of active Treg (aTreg) cells without altering Treg abundance or identity. Mechanistically, PRC1. 1 deposits H2AK119 monoubiquitylation (H2AK119ub1) at active promoters, enabling rather than repressing transcriptional activation of aTreg programs. Disruption of PRC1. 1 via Kdm2b ablation or pharmacological inhibition with iBP, a selective inhibitor, reduces H2AK119ub1, blunts stimulus-dependent transcriptional activation, and suppresses Treg activation. Notably, Treg-specific Kdm2b deletion in melanoma-bearing mice enhances anti-tumor immunity and synergizes with anti-PD-L1 therapy. Therefore, our study underscores H2AK119ub1 as a dual-function epigenetic mark and PRC1. 1 as a molecular rheostat fine-tuning Treg adaptability, establishing PRC1. 1 as a therapeutic target to decouple immune suppression in cancer while preserving Treg homeostasis.

Concepts Keywords
Deletion Animals
H2ak119ub1 Cell Plasticity
Homeostasis F-Box Proteins
Immunosuppressive F-Box Proteins
Promoters H2AK119ub1
Histones
Histones
Humans
Melanoma, Experimental
Mice
Mice, Knockout
plasticity
Polycomb
Polycomb Repressive Complex 1
Polycomb Repressive Complex 1
PRC
T-Lymphocytes, Regulatory
Treg
tumor-infiltrated Treg
Ubiquitination

Semantics

Type Source Name
disease MESH melanoma
pathway KEGG Melanoma
disease MESH tumor
disease MESH Melanoma Experimental

Original Article

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