The gut dysbiosis and plasma lipid metabolisms signatures in children with active tuberculosis

Publication date: Jul 09, 2025

Linear discriminant effect size analysis (LEfSe) was performed to obtain a linear discriminant analysis (LDA) score histogram .Statistical analysis was performed using R (version 4. 3.1). The alpha diversity was evaluated using the Shannon index and Observed species index, and compared between groups using the Wilcoxon rank-sum test. Normality test of the data was performed using the Shapiro-Wilk test, and non-parametric data are presented as the median and interquartile range. Significant differences in community composition were determined using permutational multivariate analysis of variance (PERMANOVA). Based on the distribution, either a Students t-test (for normally distributed data) or a Wilcoxon rank-sum test (for non-normally distributed data) was used to compare two groups. Samples were then vortexed (10 min intervals, 30 min at room temperature) and processed using a filter screen (70 m). To evaluate the risk of clinical comorbidities in children with TB, binary logistic regression analysis was performed, comparing TB patients with non-TB infectious disease controls.

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Original Article