Comparison of Two MPTP Doses on Mouse Behaviors and Pathologies.

Publication date: Jul 15, 2025

Parkinson’s disease (PD), a prevalent neurodegenerative disorder, is characterized by the selective and progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta, the presence of Lewy bodies (LBs) within neurons, and gliosis. The mouse model induced by the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is one of the most commonly utilized animal models for PD; however, its ability to accurately replicate the full spectrum of motor and non-motor symptoms remains contentious. In this study, we employed novel MPTP administration regimens (160 and 240 mg/kg) to examine the behavioral phenotype and pathological alterations induced by MPTP injury, utilizing a combination of behavioral, molecular, and morphological methodologies. Our findings indicate that MPTP-induced subacute PD mice exhibited a significant loss of dopaminergic neurons in the ventral midbrain, accompanied by diffuse astrogliosis and activated microglia. Nonetheless, these mice did not display other prominent movement disorders or mood abnormalities, aside from the gait disturbances associated with the administered MPTP dose. Consequently, we propose that the MPTP-induced subacute PD mouse model utilized in this study represents an early preclinical stage analogous to that observed in human PD patients.

Concepts Keywords
Molecular 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Neurodegenerative 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Nigra Animals
Parkinson Behavior, Animal
Stage behaviors
Disease Models, Animal
dopaminergic neuron
Dopaminergic Neurons
Gliosis
Male
Mice
Microglia
MPTP model
MPTP Poisoning
neuroinflammation
Parkinson Disease
Parkinson’s disease

Semantics

Type Source Name
disease MESH Parkinson’s disease
disease MESH neurodegenerative disorder
disease MESH gliosis
disease MESH movement disorders
disease MESH abnormalities
disease MESH gait
disease MESH Disease Models Animal
disease MESH MPTP Poisoning
disease MESH neuroinflammation
pathway KEGG Parkinson disease

Original Article

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