Search Results for: Pcr

June 12, 2025

A Novel Multiplex qPCR Platform for Detection of Mycobacterium tuberculosis Drug Resistance Using Synthetic Mutation Standards and Fluorescence Quenching Probes

Tuberculosis News

Publication date: Jun 10, 2025 The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis strains has intensified the need for rapid, accurate, and affordable molecular diagnostics. In this ... Read more

June 6, 2025June 6, 2025

Development and validation of novel RT-PCR assay for molecular diagnostic of viral variants using SARS-CoV-2 as a case study.

Coronavirus Literature

Publication date: Aug 01, 2025 Emerging viruses have long posed significant challenges to global public health, frequently leading to widespread morbidity and mortality. The ongoing evolution of viruses driven by ... Read more

June 1, 2025

varVAMP: degenerate primer design for tiled full genome sequencing and qPCR.

Coronavirus Literature

Publication date: May 31, 2025 Time- and cost-saving surveillance of viral pathogens is achieved by tiled sequencing in which a viral genome is amplified in overlapping PCR amplicons and qPCR. ... Read more

May 31, 2025

Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria in smear-negative sputum by a multiplex PCR assay: a clinical feasibility study.

Tuberculosis Literature

Publication date: May 30, 2025 Accurate and rapid differentiation of Mycobacterium tuberculosis (M. tuberculosis) and nontuberculous mycobacteria (NTM) remains challenging, especially in smear-negative samples. Routine diagnostic methods often lack specificity ... Read more

May 28, 2025

Evaluation of direct-to-PCR (D2P) method for molecular diagnosis of infectious diseases.

Coronavirus Literature

Publication date: May 26, 2025 This study evaluates the performance of the Direct-to-PCR (D2P) method as a streamlined, extraction-independent alternative to conventional nucleic acid extraction techniques for diagnosing urinary tract ... Read more

May 24, 2025May 24, 2025

Identification of Nontuberculous Mycobacteria in Patients with Multidrug-resistant Tuberculosis in Quezon City, Philippines, Using Multiplex PCR.

Tuberculosis Literature

Publication date: May 01, 2025 Nontuberculous mycobacteria (NTM) lung disease appears like tuberculosis infection but is resistant to primary anti-tuberculosis drugs. Hence, patients whose sputum sample tests positive for acid-fast ... Read more

May 20, 2025

Acute serum protein biomarker profile and prevalence of persistent (>6 months) neuropsychiatric symptoms in a cohort of SARS-CoV-2 PCR positive patients in Cape Town, South Africa.

Coronavirus Literature

Publication date: Jul 01, 2025 SARS-CoV-2 is a neurotrophic and pro-inflammatory virus, with several acute and more persistent neuropsychiatric sequelae reported. There are limited data from African cohorts and few ... Read more

May 19, 2025

Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples.

Coronavirus Literature

Publication date: May 17, 2025 Canine respiratory coronavirus (CRCoV) is a major contributor to the canine infectious respiratory disease complex (CIRDC). Despite its widespread prevalence, molecular assays for CRCoV detection ... Read more

May 18, 2025

Neoadjuvant treatment in advanced resectable melanoma and the need for a predictive biomarker – is pathologic complete response (pCR) the answer?

Melanoma Literature

Publication date: Jun 01, 2025 Concepts Keywords Biomarker Biomarker Complete Immunotherapy Melanoma Melanoma Neoadjuvant Neoadjuvant Pcr Pathologic complete response PD-1 Surgery Semantics Type Source Name disease MESH melanoma pathway KEGG ... Read more

May 13, 2025

Performance of a molecular beacon-based qPCR method for detection of fluoroquinolone resistance in Mycobacterium tuberculosis: A study confirmed by sequencing.

Tuberculosis Literature

Publication date: Aug 01, 2025 Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to the control and treatment of tuberculosis (TB). Rapid diagnosis of resistant strains via utilization of molecular techniques ... Read more